Figure 1

Distribution and Level of Fas in L1210/0 and L1210/DDP Cells. Expression of cell-surface Fas, leftmost panels, and intracellular Fas, right most panels in L1210/0, upper two panels, and L1210/DDP cells, lower two panels. The levels of cell surface Fas (dark lines) were determined using fluorochrome conjugated anti-Fas antibodies (Pharmingen Inc.) and flow cytometry. The levels of intracellular Fas were determined subsequent to cellular permeabilization and fixation. The Fas levels are measured relative to staining for fluorochrome-conjugated isotype control (grey lines).