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Table 2 Detection of antibodies against γ1TCR-I and γ1TCR-II in vaccinated Lewis rats

From: Antigenized antibodies expressing Vβ8.2 TCR peptides immunize against rat experimental allergic encephalomyelitis

    

Days After Vaccination

a

Immunogen

Rats (No.)

Responders (No.)

0

21

28

50

 

γ1TCR-I

6

6/6

≤2.3*

3.9 ± .2

4.2 ± .2

4.5 ± 0.2

 

γ1TCR-II

10

10/10

≤2.3

3.7 ± 0.2

4.1 ± 0.1

4.5 ± 0.2

 

γ1WT

10

10/10

≤2.3

3.2 ± 0.4

3.9 ± 0.2

4 ± 0.2

 

CFA

5

0/5

≤2.3

≤2.3

2.6

≤2.3

 

-

6

0/6

-

-

≤2.3

≤2.3

b

       
 

γ1TCR-I

6

6/6

≤2.3

4 ± 0.2

4 ± 0.2

4.6 ± 0.3

 

γ1TCR-II

10

10/10

≤2.3

4.1 ± 0.3

4.4 ± 0.3

4.7 ± 0.5

 

γ1WT

10

10/10

≤2.3

3.3 ± 0.3

4 ± 0.2

4.2 ± 0.2

 

CFA

5

0/5

≤2.3

≤2.3

≤2.3

≤2.3

 

-

6

0/6

-

-

≤2.3

≤2.3

  1. * Antibody titers are expressed in log10. Sera were tested on microtiter plates coated with each of the TCR antigenized antibody γ1TCR-I (panel a) or γ1TCR-II (pane b). End point dilutions were determined as the last serum dilution binding with an OD ≥ 0.200.