Figure 4

iDCs loaded with Hsp70 or Hsp70-PC from MDA-MB-231 cells can prime human lymphocytes to respond to MDA-MB-231 cell extracts. Purified Hsp70, Hsp70-PCs from MDA-MB-231 cells or soluble protein extracts from MDA-MB-231 tumour cells (TC) were incubated with iDCs isolated from two healthy female blood donors (Donor A; grey bars. Donor B; black bars). The iDCs were incubated [first stimulation (1)] with CD14^- PBMCs from the same donor. The cells were cultured for 10 days and then, re-incubated [second stimulation (2)] with iDCs from the same donor loaded with TC. Supernatants were tested for IFN-γ (pg/mL) production by ELISA. The label 'Hsp70-PC(1)/TC(2)' in this figure refers to IFN-γ production by CD14^-PBMCs following first and second incubations with iDCs loaded with Hsp70-PC and TC respectively. Other labels follow a similar paradigm.