Bacterial challenge (CFU)
|
106
|
107
|
108
|
---|
Time
|
36 hrs post infection
|
18 hrs post infection
|
36 hrs post infection
|
18 hrs post infection
|
36 hrs post infection
|
---|
Control group (non-immunized)
|
0
|
1.08 ± 0.14 × 106
|
8.98 ± 1.94 × 106
|
1.11 ± 0.20 × 108
|
2N/A
|
PBS
|
0
|
4.92 ± 0.94 × 105
|
1.54 ± 0.94 × 106
|
3.16 ± 1.94 × 106
|
2N/A
|
IFA
|
0
|
2.35 ± 1.12 × 105
|
1.23 ± 1.14 × 106
|
1.80 ± 0.22 × 106
|
2.16 ± 1.10 × 106
|
PHIS-01
|
0
|
3.64 ± 1.44 × 105
|
1.13 ± 2.31 × 106
|
8.90 ± 3.20 × 105
|
5.60 ± 0.94 × 105
|
PHYTOL
|
0
|
4.0 ± 0.25 × 103
|
0
|
8.90 ± 1.12 × 104
|
0
|
- 1 Peritoneal fluid samples from all groups of mice were diluted in LB medium (1:200), and 10 μl of diluted samples were streaked on agar plates using calibrated loops. CFUs were scored 18 and 36 hours following bacterial challenge and recorded as CFU/ml. The results represent the average of two experiments (n = 4 for each group) ± SEM. 2 N/A: Not assessable since all mice were dead within 18 hrs.