Phytol-based novel adjuvants in vaccine formulation: 2. assessment of efficacy in the induction of protective immune responses to lethal bacterial infections in mice

Table 2 Recovery of S. aureus from peritoneal fluid obtained from mice challegened with live S. aureus

Bacterial challenge (CFU)	10⁶	10⁷		10⁸
Time	36 hrs post infection	18 hrs post infection	36 hrs post infection	18 hrs post infection	36 hrs post infection
Control group (non-immunized)	0	1.08 ± 0.14 × 10⁶	8.98 ± 1.94 × 10⁶	1.11 ± 0.20 × 10⁸	²N/A
PBS	0	4.92 ± 0.94 × 10⁵	1.54 ± 0.94 × 10⁶	3.16 ± 1.94 × 10⁶	²N/A
IFA	0	2.35 ± 1.12 × 10⁵	1.23 ± 1.14 × 10⁶	1.80 ± 0.22 × 10⁶	2.16 ± 1.10 × 10⁶
PHIS-01	0	3.64 ± 1.44 × 10⁵	1.13 ± 2.31 × 10⁶	8.90 ± 3.20 × 10⁵	5.60 ± 0.94 × 10⁵
PHYTOL	0	4.0 ± 0.25 × 10³	0	8.90 ± 1.12 × 10⁴	0

¹ Peritoneal fluid samples from all groups of mice were diluted in LB medium (1:200), and 10 μl of diluted samples were streaked on agar plates using calibrated loops. CFUs were scored 18 and 36 hours following bacterial challenge and recorded as CFU/ml. The results represent the average of two experiments (n = 4 for each group) ± SEM. ² N/A: Not assessable since all mice were dead within 18 hrs.