Table 1 Neutralizing antibody titer (NT titer) (geometric mean) of sera from immunized mice against heterologous H5N1 influenza virus (A/Thailand/1/RPNP/2005). Sera from 5 groups of 5 mice per recombinant protein immunization were collected 1 week after last immunization with 250 μg of recombinant HA or NA, or NS1 or M proteins. Mann-Whitney method
Recombinant proteins | Neutralization titer | ELISA titer | ||
|---|---|---|---|---|
GMT (± SD) | P | GMT (± SD) | P | |
NA/COS-7 | 34 (± 8) | 0.36 | 160 (± 78) | 0.01 |
NA/E. coli | 13 (± 5) | 60 (± 21) | ||
HA/COS-7 | 91.8 (± 35.7) | 0.01 | 422 (± 175) | 0.28 |
HA/E. coli | 22 (± 8) | 160 (± 87) | ||
NS1/COS-7 | 139* (± 35) | 0.01 | 1688* (± 701) | 0.01 |
NS1/E. coli | 22 (± 8) | 422 (± 175) | ||
M/COS-7 | 0 | NA | 40 (± 21) | 0.1 |
M/E. coli | 0 | 22 (± 8) | ||