Figure 3

Induction of a T regulatory phenotype in CTCL cells. Untreated Day 0 CTCL cells were stained for co-expression of CD4 on the cell surface and CTLA-4 in the cytoplasm. DC were fed viable CTCL in ratio of > 10:1 or apoptotic CTCL cells in ratios that exceeded 10 viable (B, D, & F) or apoptotic CTCL cells (C, E, & G):1 DC and cultured overnight. Fresh responding CTCL cells were added on Day 1 and subsequently co-cultured. On Day 2, co-expression of: B & C membrane CD4 and cytoplasmic CTLA4; D & E membrane CD4 and membrane CD25; or F & G cytoplasmic expression of FoxP3 were measured. Results are representative of 5 separate experiments performed on cells isolated from cultures of 5 different CTCL patients. Apoptotic = CD3-treated, Viable = CD4-treated