Intracellular detection of IL-12 and COX-2 in MDDC. The cells were stimulated (or not) and processed for flow cytometry analysis as described in Methods.A. Dot plots in this panel show MDDC, gated on CD209+cells that express intracellular IL-12 in unstimulated and IFNγ+LPS-stimulated immature MDDC from fresh PBMC. B. Dot plots in this panel show intracellular staining of COX-2 in unstimulated and LPS stimulated immature MDDC from fresh PBMC.