Phenotypic analysis of DC purity in non-matured DC cultures from roller bottle and static flask cultures. DC were generated as before in 850 cm2 roller bottles or T-175 static flasks for 4 days and then incubated for an additional 24 h without any additional cytokines ("Not matured"). The floating cells were harvested after this additional 24 h incubation and stained for CD11c, CD13, CD14, CD83 and CD86 and analyzed by flow cytometry. In each case all the isolated floating cells were analyzed without gating and phenotype of DC compared between the roller bottles and static flask system. The numbers in the dot plots indicate the percentage of cells out of the total population of floating cells having the indicated phenotype. The results of one out of 4 similar experiments are shown.