Serum CXCL9, CXCL10 and CXCL11 concentration in the IC patients. Panel A: Sera from IC patients (n = 32) and normal, healthy donors (n = 16) were isolated and evaluated for the presence of CXCR3 ligands by ELISA, capable of detecting >10 pg/ml of each CXCR3 ligand. The data presented are the mean CXCL9, CXCL10, and CXCL11 of IC patient and normal healthy donors concentrations ± SEM. Asterisks (⋆) indicate statistically significant differences, i.e., p < 0.01, between the healthy donors and IC patients. Panel B: Control or anti-CXCL10 Ab solutions were administered 2 days prior to CYP challenge and every 2 days thereafter. Five days after CYP administration, the serum levels of CXCL9, CXCL10, and CXCL11 were determined by ELISA. The data presented are the mean concentrations ± SEM in each group. Asterisks (⋆) indicate statistically significant (p < 0.01) differences between unaffected and CYP-induced groups. Triangles (▲) indicate statistically significant (p < 0.01) differences between control Ab- and anti-CXCL10 Ab-treated groups administered CYP.