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Figure 4 | Journal of Immune Based Therapies and Vaccines

Figure 4

From: Evaluation of recombinant invasive, non-pathogenic Eschericia coli as a vaccine vector against the intracellular pathogen, Brucella

Figure 4

Microfluorimetry of supernatant of HeLa cells expressing murine IL12 indicate efficient plasmid delivery after infection by recombinant invasive E. coli vaccine. FACS analysis showed greater than 70% of HeLa cells were expressing murine specific IL12 at 72 h after 3 h infection with the invasive E. coli vaccine. The positive control was endogenous IL12 produced by the mouse macrophage cell line RAW 264.7. Negative controls included invasive E.coli not carrying the murine IL12 expression vector (E. coli BL21 infected HeLa supernatant) and uninfected HeLa cells supernatant.

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