Figure 6
DCPs derived from BM HPCs of tumor-bearing mice suppress tumor growth in vivo. (A) Ex vivo expansion of DCPs from BM HPCs of tumor-bearing Bulb/c mice. Balb/c mice were injected with CT26/optE6E7 tumor cells, and after 14 days, BM HPCs (Sca1+/Lin-) were harvested and cultured on LSC-KFT cells. After ten days, the expanded HPCs were transferred to LSC-KFT-mGM15 to generate DCPs. A representative mouse DCP expansion growth curve is shown. (B) Suppression of tumor growth in Balb/c mice immunized with LV-modified DCPs. CT27/optE6E7 tumor cells were first established in Balb/c mice. The mice were immunized with the ex vivo expanded DCPs, which were derived from the BM HPCs of CT26/optE67 tumor-bearing Balb/c mice. Two types of LV-modified DCs were tested: DCs transduced either by LV-optE6E7 alone or by LV-optE6E7 plus LV-calnexin. The percentages of survival of the two groups of mice are illustrated. Survival was based on tumor size smaller than 1 cm3 without lesions.