Assessment of spleen cell phenotype in STZ injected mice after administration of DNA-HSP65. Five weeks after the STZ injections, 5 × 106 spleen cells were labeled with anti-CD4, anti-CD8, anti-CD25, anti-CD103 and anti-CTLA-4 mAbs for fluorescence-activated cell sorter analysis. The results were expressed in total number of cells per spleen for CD4+ (A), CD8+ (B) T cells and CD4+CD25+CD103+ (C) and CD4+CD25+CTLA-4+ (D) T regulatory cells. Statistical significance was determined by ANOVA followed by Tukey's test. *p < 0.05 versus control group; #p < 0.05 versus STZ groups.