Figure 3

Cytokine profile of innate immune cells shortly after i.n. immunisation. Isolated NALT and CLN cells were stimulated for 6 h with BD Leukocyte Activation Cocktail plus GolgiPlug (or GolgiPlug alone, data not shown), stained with surface mAb to determine CD11c, F4/80 and Ly6G populations and then permeabilised and stained with anti-cytokine flurochrome-labelled mAb. Data represent percent of cytokine positive cells out of total cell population ± SD. The * indicates significant values of p < 0.05; **, p < 0.01; ***, p < 0.001, as determined by one-way ANOVA followed by Bonferroni's multiple comparison test compared to naïve mice (i.e. 0 h).