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Table 2 Percentages of innate populations within the NALT and CLN after i.n. immunisation.

From: Probing local innate immune responses after mucosal immunisation

Tissue Cell Type 0 h 5 h 24 h 72 h
NALT DC 6.5 ± 0.6 5.4 ± 0.5 7.8 ± 0.8* 6.7 ± 0.5
  7.6 ± 0.8 4.9 ± 0.4*** 7.6 ± 0.9 7.9 ± 1.1
  PMN 6.9 ± 1.1 4.9 ± 0.4** 5.9 ± 0.8 8.0 ± 1.2
CLN DC 4.1 ± 0.3 4.3 ± 0.3 2.4 ± 0.2*** 5.5 ± 0.6***
  3.6 ± 0.3 3.2 ± 0.2 3.7 ± 0.4 6.7 ± 0.7***
  PMN 3.5 ± 0.3 5.5 ± 0.9*** 3.2 ± 0.3 2.1 ± 0.2**
  1. Cells were isolated from NALT and CLN of Balb/c mice 5, 24 and 72 h after i.n. immunisation with PBS (naïve mice) or LT + Ag85B-ESAT6, and stained with flurochrome-labelled mAb and analysed by flow cytometry in which 20,000-200,000 events were recorded. Numbers are mean percentage of total cells ± SD. * indicates significant values of p < 0.05; **, p < 0.01; ***, p < 0.001, as determined by one-way ANOVA followed by Bonferroni's multiple comparison test compared to naïve mice (i.e. 0 h).